mouse anti-β3 integrin subunit antibody  (Millipore)

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Identification of amino acids essential for the antiangiogenic activity of tumstatin and its use in combination antitumor activity

doi: 10.1073/pnas.0807055105

Figure Lengend Snippet: Tumstatin interaction with αvβ3 integrin. 3D modeling of the potential interaction between tumstatin and αvβ3 integrin and surface diagram of the ligand binding site. The αv and the β3-domains of αvβ3 integrin are shown as beige and gray surfaces, respectively. The noncompetitive RGD peptide is a magenta stick model. The tumstatin peptide region of tumstatin is shown in yellow, and the LVD amino acids of tumstatin peptide are green sticks. The manganese atoms interacting with the RGD and tumstatin peptides are shown as cyan spheres. The surfaces were calculated with the program GRASP (13) and used as input for Povscript+ (14). The final images were ray-traced in POV-Ray (www.povray.org). (Inset) Diagram illustration of the tumstatin peptide interaction with the β3 integrin subunit of αvβ3 integrin. The amino acids of the β3 integrin subunit that interact with the manganese atom are shown as sticks colored blue, whereas the backbone of β3 integrin is depicted in gray. The tumstatin peptide is shown in yellow, with the LVD amino acids colored green. D (84) of tumstatin peptide is shown to interact with the manganese atom in the β3 subunit of αvβ3 integrin.

Article Snippet: For comparative integrin labeling of SP and C-PAE cells, the above staining procedure was employed using monoclonal mouse anti-human αv integrin subunit or αvβ3 integrin antibodies (Chemicon), hamster anti-mouse β3 integrin subunit antibody (BD PharMingen), and corresponding fluorescent IgG secondary antibodies (Jackson Laboratories).

Techniques: Ligand Binding Assay